Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol.
نویسندگان
چکیده
A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 +/- 0.66 ng/ml (mean +/- S.D.).
منابع مشابه
Plasma Levels of 3-Methoxy-4-Hydroxyphenylglycol and Relapse of Major Depressive Episode after Treatment with Milnacipran: One-Year Follow-Up Study
Citation: Yoshimura R, Katsuki A, Atake K, Hori H, Igata R, et al. (2016) Plasma Levels of 3-Methoxy-4-Hydroxyphenylglycol and Relapse of Major Depressive Episode after Treatment with Milnacipran: One-Year Follow-Up Study. J Depress Anxiety 6: 254. doi:10.4172/2167-1044.S1-024 4 Copyright: © 2016 Yoshimura R, et al. This is an open-access article distributed under the terms of the Creative Comm...
متن کاملFree 3-methoxy-4-hydroxyphenylglycol determined in plasma by liquid chromatography with coulometric detection.
We describe a simple "high-performance" liquid-chromatographic method for determining free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma, with coulometric detection and with 4-methoxy-3-hydroxyphenylglycol (iso-MHPG) as the internal standard. MHPG and iso-MHPG are extracted from plasma into ethyl acetate and the extract is washed with a sodium bicarbonate solution, evaporated, reconstituted,...
متن کاملEstrogen raises the sweating threshold in postmenopausal women with hot flashes.
OBJECTIVE To determine if estrogen ameliorates hot flashes by raising the core body temperature sweating threshold, by reducing core body temperature fluctuations, and/or by reducing sympathetic activation (as measured by plasma 3-methoxy-4-hydroxyphenylglycol). DESIGN Laboratory physiological study. SETTING University medical center. PATIENT(S) Twenty-four healthy postmenopausal women re...
متن کاملCan Plasma Level of 3-methoxy-4-hydroxyphenylglycol Predict the Response for Selective Serotonin Reuptake Inhibitor and Serotonin Noradrenaline Reuptake Inhibitor in Major Depressive Disorder?
Copyright: © 2017 Yoshimura R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Can Plasma Level of 3-methoxy-4-hydroxyphenylglycol Predict the Response for Selective Serotonin Reuptake Inhibitor and Ser...
متن کاملDuloxetine, a Selective Noradrenaline Reuptake Inhibitor, Increased Plasma Levels of 3-Methoxy-4-hydroxyphenylglycol but Not Homovanillic Acid in Patients with Major Depressive Disorder
OBJECTIVE We investigated the effects of duloxetine on the plasma levels of catecholamine metabolites and serum brain-derived neurotrophic factor (BDNF) in 64 patients with major depressive disorder (MDD). METHODS Major depressive episode was diagnosed using the Structured Clinical Interview for the Diagnostic and Statistical Manual of Mental Disorders-fourth edition (DSM-IV) according to the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of chromatography
دوره 377 شماره
صفحات -
تاریخ انتشار 1986